Publications

Publications

Becker, A., Chen, X., Dresselhaus, T., Gutsche, N., Müller-Schüssele, S.J., Sprunck, S., Thei?en, G., de Vries, S., Zachgo, S. (2025), Sexual reproduction in land plants: an evolutionary perspective, Plant Reprod. 38,12.

Sugi, N., Calhau, A.R.M., Jacquier, N.M.A., Millan-Blanquez, M., Becker, J.D. Begcy, K., Berger, F., Bousquet-Antonelli, C., Bouyer, D., Cai, G., Cheung, A.Y., Coimbra, S., Denninger, P., Dresselhaus, T., Feijó, J.A., Fowler, J.E., Geelen, D., Grossniklaus, U., Higashiyama, T., Honys, D., Igawa, T., Ingram, G., Jaillais, Y., Johnson, M.A., Kato, M., Kawachi, M., Kawashima, T., Kim, Y.J., Li, H.J., Mongrand, S., Motomura, K., Nagahara, S., Nakajima, K.P., Nelms, B., Qu, L.J., Schnittger, A., Schoolten, S., Sprunck, S., Sun, M.X., Twell, D., Weijers, D., Yang, W.C., Maruyama, D., Widiez, T. (2024). The peri-germ cell membrane: poorly characterized but key interface for plant reproduction. Nat Plants 10(11):1607-1609. doi: 10.1038/s41477-024-01818-5.

Wang, W., Malka, R., Lindemeier, M., Cyprys, P., Tiedemann, S., Sun, K., Zhang, X., Xiong, H., Sprunck, S., Sun, M.X. (2024). EGG CELL 1 contributes to egg-cell-dependent preferential fertilization in Arabidopsis. Nat Plants 10(2):268-282. doi: 10.1038/s41477-023-01616-5.

Liu, L., Trendel, J., Jiang, G., Liu, Y., Bruckmann, A., Küster, B., Sprunck, S., Dresselhaus, T., Bleckmann, A. (2023). RBPome identification in egg-cell like callus of Arabidopsis. Biol Chem. 404(11-12):1137-1149. doi: 10.1515/hsz-2023-0195.

Sprunck, S., Schwechheimer, C., Morita, M.T. (2022) Editorial overview: Cell biology and cell signalling. Corr Opin Plant Biol. 2022, 70:102312. doi: 10.1016/j.pbi.2022.102312.

Wang, W., Xiong, H., Cyprys, P., Malka, R., Flores-Tornero, M., Zhao, P., Peng, X., Sprunck, S., Sun, M.-X. (2022) DMP8 and 9 regulate HAP2/GCS1 trafficking for the timely acquisition of sperm fusion competence. PNAS 119, 45, e2207608119.

Flores-Tornero, M., Sprunck, S., Dresselhaus T. (2021) Identification and characterization of reproductive mutations in Arabidopsis. Meth. Mol. Biol. 2200, 371-390.

Flores-Tornero, M., Wang, L., Pote?il, D., Hafidh, S., Vogler, F., Zdráhal, Z., Honys, D., Sprunck, S., Dresselhaus, T. (2021) Comparative analyses of angiosperms secretomes identify apoplastic pollen tube functions and novel secreted peptides. Plant Reprod. 34, 47-60.

Flores-Tornero, M., Vogler, F., Mutwil, M., Potě?il, D., Ihnatová, I., Zdráhal, Z., Sprunck, S., Dresselhaus, T. (2020) Transcriptomic and proteomic insights into Amborella trichopoda male gametophyte functions. Plant Physiol. doi: 10.1104/pp.20.00837.
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Kulich, I., Vogler, F., Bleckmann, A., Cyprys, P., Lindemeier, M., Fuchs, I., Krassini, L., Schubert, T., Steinbrenner, J., Beynon, J., Falter-Braun, P., L?ngst, G., Dresselhaus, T., Sprunck, S. (2020) ARMADILLO REPEAT ONLY proteins confine Rho GTPase signalling to polar growth sites. Nat. Plants 6, 1275-1288.
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Mergner, J., Frejno, M., List, M., Papacek, M., Chen, X., Chaudhary, A., Samaras, P., Richter, S., Shikata, H., Messerer, M., Lang, D., Altmann, S., Cyprys, P., Zolg, D.P., Mathieson, T., Bantscheff, M., Hazarika, R.R., Schmidt, T., Dawid, C., Dunkel, A., Hofmann, T., Sprunck, S., Falter-Braun, P., Johannes, F., Mayer, K.F.X., Jürgens, G., Wilhelm, M., Baumbach, J., Grill, E., Schneitz, K., Schwechheimer, C., Küster, B. (2020) Mass-spectrometry-based draft of the Arabidopsis proteome. Nature 579: 409–414.
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Sprunck, S. (2020) Twice the fun, double the trouble: gamete interactions in flowering plants. Curr. Opin. Plant Biol. 53: 106-116. ????? ? ???

Bauer, N., ?kiljaica, A., Malenica, N., Razdorov, G., Klasi?, M., Jurani?, M., Mo?ibob, M., Sprunck, S., Dresselhaus, T., Leljak Levani?, D. (2019) The MATH-BTB protein TaMAB2 accumulates in ubiquitin-containing foci and interacts with the translation initiation machinery in Arabidopsis. Front Plant Sci. 10:1469.

Cyprys, P., Lindemeier, M., Sprunck, S. (2019) Gamete fusion is facilitated by two sperm cell-expressed DUF679 membrane proteins. Nat Plants. 5, 253-257. doi: 10.1038/s41477-019-0382-3.

Ehrnsberger, H.F., Pfaff, C., Hachani, I., Flores-Tornero, M., S?rensen, B.B., L?ngst, G., Sprunck, S., Grasser, M. and Grasser, K.D. (2019)?The UAP56-interacting export factors UIEF1 and UIEF2 function in mRNA export. Plant Physiol.?179, 1525-1536.

Flores-Tornero, M., Proost, S., Mutwil, M., Scutt, C.P., Dresselhaus, T., Sprunck, S. (2019) Transcriptomics of?manually isolated Amborella trichopoda egg apparatus cells. Plant Reprod. 32, 15-27.

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Sprunck, S., Urban, M., Strieder, N., Lindemeier, M., Bleckmann, A., Evers, M., Hackenberg, T., M?hle, C., Dresselhaus, T., Engelmann, J.C. (2019) Elucidating small RNA pathways in Arabidsopsis thaliana egg cells. bioRxiv, doi.org/10.1101/525956.

Kirioukhova, O., Shah, J.N., Larsen, D.S., Tayyab, M., Mueller, N.E., Govind, G., Baroux, C., Federer, M., Gheyselinck, J., Barrell, P.J., Ma, H., Sprunck, S., Huettel, B., Wallace, H., Grossniklaus, U., Johnston, A.J. (2018) Aberrant imprinting may underlie evolution of parthenogenesis. Sci Rep. 8, 10626.doi.org/10.1101/525956

Pfaff, C., Ehrnsberger, H.F., Flores-Tornero, M., S?rensen, B.B., Schubert, T., L?ngst, G., Griesenbeck, J., Sprunck, S., Grasser, M. and Grasser, K.D. (2018) ALY RNA-binding proteins are required for nucleo-cytosolic mRNA transport and modulate plant growth and development in Arabidopsis. Plant Physiol. 177, 226-240.

Chen, J., Strieder, N., Krohn, N.G., Cyprys, P., Sprunck, S., Engelmann, J.C. and Dresselhaus, T. (2017) Zygotic Genome Activation Occurs Shortly After Fertilization in Maize. Plant Cell, 29, 2106-2125.

Englhart, M., ?olji?, L. and Sprunck, S. (2017) Manual Isolation of Living Cells from the Arabidopsis thaliana Female Gametophyte by Micromanipulation. Methods Mol Biol., 1669, 221-234.

Resentini, F, Cyprys, P., Steffen, J.G., Alter, S., Morandini, P.A., Mizzotti, C., Lloyd, A., Drews, G.N., Dresselhaus, T., Colombo, L., Sprunck, S. and Masiero, S. (2017) SUPRESSOR OF FRIGIDA (SUF4) supports gamete fusion via regulating Arabidopsis EC1 gene expression. Plant Physiol. 173, 155-166.

Dresselhaus, T., Sprunck, S. and Wessel, G.M. (2016) Fertilization mechanisms in flowering plants. Curr. Biol. 26, R125-R139.

Hauptmann, J., Schraivogel, D., Bruckmann, A., Manickavel, S., Jakob, L., Eichner, N., Pfaff J., Urban, M., Sprunck, S., Hafner, M., Tuschl, T., Deutzmann, R. and Meister, G. (2015) Biochemical isolation of Argonaute protein complexes by Ago-APP. Proc. Natl. Acad. Sci. USA 112, 11841-11845.

Sprunck, S. and Dresselhaus, T. (2015) Three cell fusions during double fertilization. Cell 161, 708-709.

Vogler, F., Konrad, S.S.A. and Sprunck, S. (2015) Knockin' on pollen's door: live cell imaging of early polarization events in germinating Arabidopsis pollen. Front Plant Sci. 6, 246.

Vogler, F. and Sprunck, S. (2015) F-actin forms mobile and unwinding ring-shaped structures in germinating Arabidopsis pollen expressing Lifeact. Plant Signal Behav. 10, e1075684.

Denninger, P., Bleckmann, A., Lausser, A., Vogler, F., Ott, T., Ehrhardt, D.W., Frommer, W.B., Sprunck, S., Dresselhaus, T. and Grossmann, G. (2014) Male–female communication triggers calcium signatures during fertilization in Arabidopsis. Nature Comm. 5, 4645.

Pereira, A.M., Masiero, S., Nobre, M.S., Costa, M.L., Solís, M.T., Testillano, P.S., Sprunck, S. and Coimbra, S. (2014) Differential expression patterns of Arabinogalactan Proteins in Arabidopsis thaliana reproductive tissues. J. Exp. Bot. 65, 5459-5471.

Sprunck, S., Hackenberg, T., Englhart, M. and Vogler, F. (2014) Same same but different: sperm-activating EC1 and ECA1 gametogenesis-related family proteins. Biochem. Soc. Trans. 42, 401-407.

Vogler, F., Schmalzl, C., Englhart, M., Bircheneder, M. and Sprunck, S. (2014) Brassinosteroids promote Arabidopsis pollen germination and growth. Plant Reprod. 27, 153-167.

Leljak-Levani?, D., Jurani?, M. and Sprunck, S. (2013) De novo zygotic transcription in wheat (Triticum aestivum L.) includes genes encoding small putative secreted peptides and a protein involved in proteasomal degradation. Plant Reprod. 26, 267-285.

Rademacher, S. and Sprunck, S. (2013) Downregulation of egg cell-secreted EC1 is accompanied with delayed gamete fusion and polytubey. Plant Signal. Behav. 8, e27377.

Dresselhaus, T. and Sprunck, S. (2012) Plant fertilization: maximizing reproductive success. Curr. Biol. 22, R487-R489.

Juranic, M., Srilunchang, K.-o., Krohn, N.G., Leljak-Levanic, D., Sprunck, S. and Dresselhaus, T. (2012) Germline-specific MATH-BTB substrate adaptor MAB1 regulates spindle length and nuclei identity in maize. Plant Cell 24, 4974-4991.

Sprunck, S., Rademacher, S., Vogler, F., Gheyselinck, J., Grossniklaus, U. and Dresselhaus, T. (2012) Egg cell–secreted EC1 triggers sperm cell activation during double fertilization. Science 338, 1093-1097.

V?lz, R., von Lyncker, L., Baumann, N., Dresselhaus, T., Sprunck, S. and Gro?-Hardt, R. (2012) LACHESIS-dependent egg-cell signaling regulates the development of female gametophytic cells. Development 139, 498-502.

Zhao, X., Harashima, H., Dissmeyer, N., Pusch, S., Weimer, A.K., Bramsiepe, J., Bouyer, D., Rademacher, S., Nowack, M.K., Novak, B., Sprunck, S., and Schnittger, A. (2012) A general G1/S-phase cell-cycle control module in the flowering plant Arabidopsis thaliana. PLoS Genet. 8, e1002847.

Sprunck, S. and Gro?-Hardt, R. (2011) Nuclear behavior, cell polarity and cell specification in the female gametophyte. Sex. Plant Reprod. 24, 123-136.

Ingouff, M., Rademacher, S., Foo, S.H., ?olji?, L., Readshaw, A., Holec, S., Xin, N., Lahouze, B., Sprunck, S. and Berger, F. (2010) Zygotic resetting of the HISTONE3 variants repertoire enables epigenetic reprogramming in Arabidopsis. Curr. Biol. 20, 2137-2143.

Sprunck, S. (2010)? Let?s get physical: gamete interaction in flowering plants. Biochem. Soc. Transact. 38, 635-640.

Ingouff, M., Sakata, T., Li, J., Sprunck, S., Dresselhaus, T. and Berger, F. (2009) The two male gametes share equal ability to fertilize the egg cell in Arabidopsis thaliana. Curr. Biol. 19, R19-R20.

Okuda, S., Tsutsui, H., Shiina, K., Sprunck, S., Takeuchi, H., Yui, R., Kasahara, R.D., Hamamura, Y., Mizukami, A., Susaki, D., Kawano, N., Sakakibara, T., Namiki, S., Itoh, K., Otsuka, K., Matsuzaki, M., Nozaki, H., Kuroiwa, T., Nakano, A., Kanaoka, M.M., Dresselhaus, T., Sasaki, N. and Higashiyama, T. (2009) Defensin-like polypeptide LUREs are pollen tube attractants secreted from synergid cells. Nature 458, 357-361.

Sprunck, S. and Dresselhaus, T. (2009) Gamete interactions during double fertilization in flowering plants. Zellbiologie aktuell 35, 22-27.

Gebert, M., Dresselhaus, T. and Sprunck, S. (2008) F-actin organisation and pollen tube growth in Arabidopsis are dependent on the gametophyte-specific Armadillo repeat protein ARO1. Plant Cell 20, 2798-2814.

Sprunck, S., Baumann, U., Edwards, K., Langridge, P. and Dresselhaus, T. (2005) The transcript composition of egg cells changes significantly following fertilization in wheat (Triticum aestivum L.). Plant J. 41, 660-672.

Dresselhaus, T. and Sprunck, S. (2003) Peptide hormone mediated signaling in plants exhibits mechanistic similarities in animals. In: Advances in Plant Physiology, Vol. 6 (Hemantarajan A. ed.). Scientific Publishers, pp. 131-177.

Amien, S., Sprunck, S., Marton, M., Cordts, S. and Dresselhaus, T. (2002) Analysis of peptide signaling in the embryo sac of maize and wheat. In: Plant Biotechnology 2002 and beyond. By I. Vasil (ed.). Kluwer Academic Publ., Dordrecht, 273-277.

Kluth, A., Sprunck, S., Becker, D., L?rz, H. and Lütticke, S. (2002) 5?deletion of a gbss1 promoter region from wheat leads to change in tissue and developmental specificities. Plant Mol. Biol. 49, 669-682.

Reinard, T., Sprunck, S., Altherr, S. and Jacobsen, H.-J. (1995) Biochemical properties of a novel cell wall protein associated with elongation growth in higher plants. J. Exp. Bot. 45, 1593-1601.

Sprunck, S., Jacobsen, H.-J. and Reinard, T. (1995) Indole-3-lactic acid is a weak auxin analogue but not an anti-auxin. J. Plant Growth Regul. 14, 191-197.